TY  - JOUR
AB  - <p>Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro. pcDNA6.2-mFas-mTNFR1- miRNA，which expresses miRNA against both mFas and mTNFR1 simultaneously，was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1-...
AU  - Dong Xi
AU  - Ming Wang
AU  - Huali Ye
AU  - Xiaoping Luo
AU  - Qin Ning
DA  - 20130101
DO  - 10.1371/journal.pone.0082330
J2  - PloS one
KW  - Sciences (General)
LA  - eng
PB  - Public Library of Science (PLoS)
PY  - 20130101
SN  - 1932-6203
SP  - e82330
T2  - PloS one
TI  - Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice
UR  - https://doaj.org/article/0261cf0e6afa456d8b627e0451aec216
Y2  - 2021-04-10T14:07:45+02:00
ER  -