TY - JOUR AB -

Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro. pcDNA6.2-mFas-mTNFR1- miRNA,which expresses miRNA against both mFas and mTNFR1 simultaneously,was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1-... AU - Dong Xi AU - Ming Wang AU - Huali Ye AU - Xiaoping Luo AU - Qin Ning DA - 20130101 DO - 10.1371/journal.pone.0082330 J2 - PloS one KW - Sciences (General) LA - eng PB - Public Library of Science (PLoS) PY - 20130101 SN - 1932-6203 SP - e82330 T2 - PloS one TI - Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice UR - https://doaj.org/article/0261cf0e6afa456d8b627e0451aec216 Y2 - 2021-04-10T14:07:45+02:00 ER -