TY  - JOUR
AB  - 5-Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of leukotrienes (LTs), biological mediators of host defense reactions and of inflammatory diseases. While the role of membrane binding in the regulation of 5-LO activity is well established, the effects of lipids on cellular activity when added to the medium has not been characterized. Here, we show such a novel function of the most abundant sulfated sterol in human blood, cholesterol sulfate (CS), to suppress LT production in human polymorphonuclear leukocytes (PMNL) and Mono Mac6 cells. We synthesized another anionic lipid, cholesterol phosphate, which demonstrated a similar capacity in suppression of LT synthesis in PMNL. Cholesteryl acetate was without effect. Cholesterol increased the effect of CS on 5-LO product synthesis. CS and cholesterol also inhibited arachidonic acid (AA) release from PMNL. Addition of exogenous AA increased the threshold concentration of CS required to inhibit LT synthesis. The effect of cholesterol...
AU  - Aleksandrov, Dmitry A
AU  - Zagryagskaya, Anna N
AU  - Pushkareva, Marina A
AU  - Bachschmid, Markus
AU  - Peters-Golden, Marc
AU  - Werz, Oliver
AU  - Steinhilber, Dieter
AU  - Sud'Ina, Galina F
DA  - 200602
DO  - 10.1111/j.1742-4658.2005.05087.x
KW  - Lipoxygenase Inhibitors ; Arachidonate 5-Lipoxygenase -- Metabolism ; Cholesterol -- Analogs & Derivatives ; Neutrophils -- Metabolism
LA  - eng
PY  - 200602
SN  - 17424658
SN  - 1742-464X
SP  - 548
T2  - The FEBS journal
TI  - Cholesterol and its anionic derivatives inhibit 5-lipoxygenase activation in polymorphonuclear leukocytes and MonoMac6 cells
Y2  - 2021-02-26T07:36:16+01:00
ER  -