TY  - JOUR
AB  - Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro. pcDNA6.2-mFas-mTNFR1- miRNA，which expresses miRNA against both mFas and mTNFR1 simultaneously，was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1-...
AU  - Xi, Dong
AU  - Wang, Ming
AU  - Ye, Huali
AU  - Luo, Xiaoping
AU  - Ning, Qin
DA  - 2013
DO  - 10.1371/journal.pone.0082330
KW  - Adenoviridae -- Genetics ; Fibrinogen -- Genetics ; Genetic Vectors -- Genetics ; Liver Failure, Acute -- Genetics ; Micrornas -- Genetics ; Receptors, Tumor Necrosis Factor, Type I -- Genetics ; Fas Receptor -- Genetics
LA  - eng
PY  - 2013
SN  - 1932-6203
SN  - 1932-6203
SP  - e82330
T2  - PloS one
TI  - Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice
Y2  - 2021-04-21T22:43:46+02:00
ER  -