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    Combined Adenovirus-Mediated Artificial microRNAs Targeting mfgl2, mFas, and mTNFR1 Protect against Fulminant Hepatic Failure in Mice (Virus-Mediated miRNA Treatment of Hepatic Failure)

    Xi, Dong, Wang, Ming, Ye, Huali, Luo, Xiaoping, Ning, Qin
    2013, Vol.8(11), p.e82330 [Peer Reviewed Journal]
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    Title: Combined Adenovirus-Mediated Artificial microRNAs Targeting mfgl2, mFas, and mTNFR1 Protect against Fulminant Hepatic Failure in Mice (Virus-Mediated miRNA Treatment of Hepatic Failure)
    Author: Xi, Dong; Wang, Ming; Ye, Huali; Luo, Xiaoping; Ning, Qin
    Contributor: Ahn, Sang Hoon (Editor)
    Subject: Research Article
    Description: Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro . pcDNA6.2-mFas-mTNFR1- miRNA,which expresses miRNA against both mFas and mTNFR1 simultaneously,was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1- miRNA was also constructed by the same procedure. Adenovirus vectors were delivered by tail-vein injection into MHV-3-infected BALB/cJ mice to evaluate the therapeutic effect. 8 of 18 (44.4%) mice recovered from fulminant viral hepatitis in the combined interference group treated with Ad-mfgl2-miRNA and Ad-mFas-mTNFR1-miRNA. But only 4 of 18 (22.2%) mice receiving Ad-mfgl2-miRNA and 3 of 18 (16.7%) mice receiving Ad-mFas-mTNFR1- miRNA survived. These adenovirus vectors significantly ameliorated inflammatory infiltration, fibrin deposition, hepatocyte necrosis and apoptosis, and prolonged survival time. Our data illustrated that combined interference using adenovirus-mediated artificial miRNAs targeting mfgl2, mFas, and mTNFR1 might have significant therapeutic potential for the treatment of fulminant hepatitis.
    Is part of: 2013, Vol.8(11), p.e82330
    Identifier: 1932-6203 (E-ISSN); 10.1371/journal.pone.0082330 (DOI)

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    Combined Adenovirus-Mediated Artificial microRNAs Targeting mfgl2, mFas, and mTNFR1 Protect against Fulminant Hepatic Failure in Mice

    Xi, Dong, Wang, Ming, Ye, Huali, Luo, Xiaoping, Ning, Qin
    PLoS ONE, 2013, Vol.8(11) [Peer Reviewed Journal]
    U.S. National Library of Medicine (NIH/NLM)
    Available
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    Title: Combined Adenovirus-Mediated Artificial microRNAs Targeting mfgl2, mFas, and mTNFR1 Protect against Fulminant Hepatic Failure in Mice
    Author: Xi, Dong; Wang, Ming; Ye, Huali; Luo, Xiaoping; Ning, Qin
    Contributor: Ahn, Sang Hoon (editor)
    Subject: Research Article
    Description: Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro . pcDNA6.2-mFas-mTNFR1- miRNA,which expresses miRNA against both mFas and mTNFR1 simultaneously,was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1-...
    Is part of: PLoS ONE, 2013, Vol.8(11)
    Identifier: 1932-6203 (E-ISSN); 10.1371/journal.pone.0082330 (DOI); 3841162 (PMCID); 24303082 (PMID)

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    Combined Adenovirus-Mediated Artificial microRNAs Targeting mfgl2, mFas, and mTNFR1 Protect against Fulminant Hepatic Failure in Mice

    Wang, Ming, Ye, Huali, Luo, Xiaoping
    PLoS One, Nov 2013, Vol.8(11), p.e82330 [Peer Reviewed Journal]
    © ProQuest LLC All rights reserved, Medical Database, Health & Medical Collection (Alumni edition), Medical Database (Alumni edition), Nursing & Allied Health Database (Alumni edition), ProQuest Pharma Collection, Health & Medical Collection, Nursing & Allied Health Database, ProQuest Agriculture Journals, Ecology Abstracts, Entomology Abstracts, Animal Behavior Abstracts, ProQuest Public Health, Materials Science Database, Advanced Technologies & Aerospace Database, Engineering Database, Biological Science Database, Publicly Available Content Database, Environmental Science Database (ProQuest), Engineering Research Database, Technology Research Database, ProQuest Nursing & Allied Health Source, ProQuest Agricultural Science Collection, ProQuest Atmospheric Science Collection, ProQuest Biological Science Collection, ProQuest Central, ProQuest Engineering Collection, ProQuest Environmental Science Collection, ProQuest Advanced Technologies & Aerospace Collection, ProQuest Hospital Collection, ProQuest Materials Science Collection, ProQuest Natural Science Collection, ProQuest Technology Collection, Hospital Premium Collection (Alumni edition), ProQuest SciTech Collection, ProQuest Health & Medical Complete, ProQuest Medical Library, Agricultural & Environmental Science Database, Earth, Atmospheric & Aquatic Science Database, Materials Science & Engineering Database, Natural Science Collection, ProQuest Central (new), ProQuest Central K-12, ProQuest Central Korea, SciTech Premium Collection, Technology Collection, Health Research Premium Collection, Health Research Premium Collection (Alumni edition), ProQuest Central Essentials, ProQuest One Academic, Environmental Science Index (ProQuest), Biological Science Index (ProQuest), Engineering Index (ProQuest), Environmental Science Collection (ProQuest), Materials Science Collection (ProQuest), Engineering Collection (ProQuest)
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    Title: Combined Adenovirus-Mediated Artificial microRNAs Targeting mfgl2, mFas, and mTNFR1 Protect against Fulminant Hepatic Failure in Mice
    Author: Wang, Ming; Ye, Huali; Luo, Xiaoping
    Subject: Muridae ; Asia ; Gene Therapy ; Hepatitis B ; Liver ; Hepatitis ; Science ; Mortality ; Cytokines ; Hospitals ; Micrornas ; Interference ; Liver Diseases ; Laboratory Animals ; Plasmids ; Gene Expression ; Tnf Inhibitors ; Medical Prognosis ; Apoptosis ; Interference
    Description: Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro. pcDNA6.2-mFas-mTNFR1- miRNA,which expresses miRNA against both mFas and mTNFR1 simultaneously,was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1-...
    Is part of: PLoS One, Nov 2013, Vol.8(11), p.e82330
    Identifier: 19326203 (E-ISSN); 10.1371/journal.pone.0082330 (DOI)

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    Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice

    Dong Xi, Ming Wang, Huali Ye, Xiaoping Luo, Qin Ning
    PloS one, 01 January 2013, Vol.8(11), p.e82330 [Peer Reviewed Journal]
    Directory of Open Access Journals (DOAJ)
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    Title: Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice
    Author: Dong Xi; Ming Wang; Huali Ye; Xiaoping Luo; Qin Ning
    Subject: Sciences (General)
    Description: Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro. pcDNA6.2-mFas-mTNFR1- miRNA,which expresses miRNA against both mFas and mTNFR1 simultaneously,was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1-...
    Is part of: PloS one, 01 January 2013, Vol.8(11), p.e82330
    Identifier: 1932-6203 (E-ISSN); 10.1371/journal.pone.0082330 (DOI)

    • Article
    Select

    Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice

    Xi, Dong, Wang, Ming, Ye, Huali, Luo, Xiaoping, Ning, Qin
    PloS one, 2013, Vol.8(11), pp.e82330 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
    Available
    More…
    Title: Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice
    Author: Xi, Dong; Wang, Ming; Ye, Huali; Luo, Xiaoping; Ning, Qin
    Subject: Adenoviridae -- Genetics ; Fibrinogen -- Genetics ; Genetic Vectors -- Genetics ; Liver Failure, Acute -- Genetics ; Micrornas -- Genetics ; Receptors, Tumor Necrosis Factor, Type I -- Genetics ; Fas Receptor -- Genetics
    Description: Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) has a poor prognosis with high in-hospital mortality. Hepatic and circulating inflammatory cytokines, such as fibrinogen like protein 2 (fgl2), FasL/Fas, and TNFα/TNFR1, play a significant role in the pathophysiology of ACLF. This study aimed to investigate the therapeutic effect of recombinant adenoviral vectors carrying constructed DNA code for non-native microRNA (miRNA) targeting mouse fgl2 (mfgl2) or both mFas and mTNFR1 on murine hepatitis virus (MHV)-3-induced fulminant hepatitis in BALB/cJ mice. Artificial miRNA eukaryotic expression plasmids against mfgl2, mFas, and mTNFR1 were constructed, and their inhibitory effects on the target genes were confirmed in vitro. pcDNA6.2-mFas-mTNFR1- miRNA,which expresses miRNA against both mFas and mTNFR1 simultaneously,was constructed. To construct a miRNA adenovirus expression vector against mfgl2, pcDNA6.2-mfgl2-miRNA was cloned using Gateway technology. Ad-mFas-mTNFR1-...
    Is part of: PloS one, 2013, Vol.8(11), pp.e82330
    Identifier: 1932-6203 (E-ISSN); 24303082 Version (PMID); 10.1371/journal.pone.0082330 (DOI)