This query concerns only resources held in libraries.
29 results
Sort by:
Add to the list:
    • Article
    Select

    Naturally occurring motility-defective mutants of Salmonella enterica serovar Enteritidis isolated preferentially from nonhuman rather than human sources

    Yim, Lucía, Betancor, Laura, Martínez, Arací, Bryant, Clare, Maskell, Duncan, Chabalgoity, José A
    Applied and environmental microbiology, November 2011, Vol.77(21), pp.7740-8 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
    Available
    More…
    Title: Naturally occurring motility-defective mutants of Salmonella enterica serovar Enteritidis isolated preferentially from nonhuman rather than human sources
    Author: Yim, Lucía; Betancor, Laura; Martínez, Arací; Bryant, Clare; Maskell, Duncan; Chabalgoity, José A
    Subject: Locomotion ; Salmonella Infections -- Microbiology ; Salmonella Infections, Animal -- Microbiology ; Salmonella Enteritidis -- Isolation & Purification
    Description: Salmonellosis represents a worldwide health problem because it is one of the major causes of food-borne disease. Although motility is postulated as an important Salmonella virulence attribute, there is little information about variation in motility in natural isolates. Here we report the identification of a point mutation (T551 → G) in motA, a gene essential for flagellar rotation, in several Salmonella enterica serovar Enteritidis field isolates. This mutation results in bacteria that can biosynthesize structurally normal but paralyzed flagella and are impaired in their capacity to invade human intestinal epithelial cells. Introduction of a wild-type copy of motA into one of these isolates restored both motility and cell invasiveness. The motA mutant triggered higher proinflammatory transcriptional responses than an aflagellate isolate in differentiated Caco-2 cells, suggesting that the paralyzed flagella are able to signal through pattern recognition receptors. A specific PCR was designed...
    Is part of: Applied and environmental microbiology, November 2011, Vol.77(21), pp.7740-8
    Identifier: 1098-5336 (E-ISSN); 21926214 Version (PMID); 10.1128/AEM.05318-11 (DOI)

    • Several versions

    Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

    Silva, Claudia, Betancor, Laura, García, Coralith, Astocondor, Lizeth, Hinostroza, Noemí, Bisio, Julieta, Rivera, Javier, Perezgasga, Lucía, Pérez Escanda, Victoria, Yim, Lucía, Jacobs, Jan, García-Del Portillo, Francisco, Chabalgoity, José A
    PLoS ONE, 2017, Vol.12(12) [Peer Reviewed Journal]

    • Several versions

    A novel prophage identified in strains from Salmonella enterica serovar Enteritidis is a phylogenetic signature of the lineage ST-1974

    D'Alessandro, Bruno, Pérez Escanda, Victoria, Balestrazzi, Lucía, Iriarte, Andrés, Pickard, Derek, Yim, Lucía, Chabalgoity, José Alejandro, Betancor, Laura
    Microbial genomics, March 2018, Vol.4(3) [Peer Reviewed Journal]

    • Article
    Select

    Repression of flagella is a common trait in field isolates of Salmonella enterica serovar Dublin and is associated with invasive human infections

    Yim, Lucía, Sasías, Sebastián, Martínez, Arací, Betancor, Laura, Estevez, Verónica, Scavone, Paola, Bielli, Alejandro, Sirok, Alfredo, Chabalgoity, José Alejandro
    Infection and immunity, April 2014, Vol.82(4), pp.1465-76 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
    Available
    More…
    Title: Repression of flagella is a common trait in field isolates of Salmonella enterica serovar Dublin and is associated with invasive human infections
    Author: Yim, Lucía; Sasías, Sebastián; Martínez, Arací; Betancor, Laura; Estevez, Verónica; Scavone, Paola; Bielli, Alejandro; Sirok, Alfredo; Chabalgoity, José Alejandro
    Subject: Flagella -- Physiology ; Salmonella Infections -- Microbiology ; Salmonella Enterica -- Pathogenicity
    Description: The nontyphoidal Salmonella enterica serovar Dublin is adapted to cattle but infrequently infects humans, very often resulting in invasive infections with high levels of morbidity and mortality. A Salmonella-induced intestinal acute inflammatory response is postulated as a mechanism to prevent bacterial dissemination to systemic sites. In S. enterica serovar Typhimurium, flagella contribute to this response by providing motility and FliC-mediated activation of pattern recognition receptors. In this study, we found 4 Salmonella enterica isolates, with the antigenic formula 9,12:-:-, that, based on fliC sequence and multilocus sequence type (MLST) analyses, are aflagellate S. Dublin isolates. Interestingly, all were obtained from human bloodstream infections. Thus, we investigated the potential role of flagella in the unusual invasiveness exhibited by S. Dublin in humans by analyzing flagellation and proinflammatory properties of a collection of 10 S. Dublin human clinical isolates. We found...
    Is part of: Infection and immunity, April 2014, Vol.82(4), pp.1465-76
    Identifier: 1098-5522 (E-ISSN); 24421045 Version (PMID); 10.1128/IAI.01336-13 (DOI)

    • Several versions

    First Release of the Bacterial Biobank of the Urban Environment (BBUE)

    Antelo, Verónica, Salazar, Cecilia, Martínez, Arací, D’alessandro, Bruno, Castro, Marta, Betancor, Laura, Barcala, María Victoria, Míguez, Diana, Gonnet, Gastón H, Iraola, Gregorio
    Microbiology Resource Announcements, 2018, Vol.7(16) [Peer Reviewed Journal]

    • Article
    Select

    Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin

    Betancor, Laura, Yim, Lucía, Martínez, Arací, Fookes, Maria, Sasias, Sebastian, Schelotto, Felipe, Thomson, Nicholas, Maskell, Duncan, Chabalgoity, José A
    The open microbiology journal, 2012, Vol.6, pp.5-13 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
    Available
    More…
    Title: Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin
    Author: Betancor, Laura; Yim, Lucía; Martínez, Arací; Fookes, Maria; Sasias, Sebastian; Schelotto, Felipe; Thomson, Nicholas; Maskell, Duncan; Chabalgoity, José A
    Subject: Comparative Genomics ; Host Specificity ; Pseudogenes ; S. Dublin ; S. Enteritidis. ; Salmonella
    Description: The Enteritidis and Dublin serovars of Salmonella enterica are closely related, yet they differ significantly in pathogenicity and epidemiology. S. Enteritidis is a broad host range serovar that commonly causes gastroenteritis and infrequently causes invasive disease in humans. S. Dublin mainly colonizes cattle but upon infecting humans often results in invasive disease.To gain a broader view of the extent of these differences we conducted microarray-based comparative genomics between several field isolates from each serovar. Genome degradation has been correlated with host adaptation in Salmonella, thus we also compared at whole genome scale the available genomic sequences of them to evaluate pseudogene composition within each serovar.Microarray analysis revealed 3771 CDS shared by both serovars while 33 were only present in Enteritidis and 87 were exclusive to Dublin. Pseudogene evaluation showed 177 inactive CDS in S. Dublin which correspond to active genes in S. Enteritidis, nine of...
    Is part of: The open microbiology journal, 2012, Vol.6, pp.5-13
    Identifier: 1874-2858 (E-ISSN); 22371816 Version (PMID); 10.2174/1874285801206010005 (DOI)

    • Several versions

    First detection of CMY-2 plasmid mediated β-lactamase in Salmonella Heidelberg in South America

    Cejas, Daniela, Vignoli, Rafael, Quinteros, Mirta, Marino, Ricardo, Callejo, Raquel, Betancor, Laura, Gutkind, Gabriel O, Radice, Marcela A
    Revista Argentina de microbiologia, 2014, Vol.46(1), pp.30-3 [Peer Reviewed Journal]

    • Several versions

    Draft Genome Sequences of Two Multidrug-Resistant Salmonella enterica Serovar Typhimurium Clinical Isolates from Uruguay

    Cordeiro, Nicolás F, D’alessandro, Bruno, Iriarte, Andrés, Pickard, Derek, Yim, Lucía, Chabalgoity, José Alejandro, Betancor, Laura, Vignoli, Rafael
    Microbiology Resource Announcements, 2018, Vol.7(4) [Peer Reviewed Journal]

    • Several versions

    A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences

    Iraola, Gregorio, Pérez, Ruben, Betancor, Laura, Marandino, Ana, Morsella, Claudia, Méndez, Alejandra, Paolicchi, Fernando, Piccirillo, Alessandra, Tomás, Gonzalo, Velilla, Alejandra, Calleros, Lucía
    BMC veterinary research, 15 December 2016, Vol.12(1), p.286 [Peer Reviewed Journal]

    • Article
    Select

    A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties

    Sasías, Sebastián, Martínez-Sanguiné, Adriana, Betancor, Laura, Martínez, Arací, D'Alessandro, Bruno, Iriarte, Andrés, Chabalgoity, José A, Yim, Lucía
    Infection and immunity, January 2018, Vol.86(1) [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
    Available
    More…
    Title: A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties
    Author: Sasías, Sebastián; Martínez-Sanguiné, Adriana; Betancor, Laura; Martínez, Arací; D'Alessandro, Bruno; Iriarte, Andrés; Chabalgoity, José A; Yim, Lucía
    Subject: Flie ; Salmonella ; Flagella ; Proinflammatory Capacity ; Serovar Dublin ; Bacterial Proteins -- Genetics ; Flagella -- Genetics ; Salmonella Enterica -- Genetics ; Sequence Deletion -- Genetics
    Description: serovar Dublin is adapted to cattle but is able to infect humans with high invasiveness. An acute inflammatory response at the intestine helps to prevent dissemination to systemic sites. Flagella contribute to this response by providing motility and FliC-mediated signaling through pattern recognition receptors. In a previous work, we reported a high frequency (11 out of 25) of Dublin isolates lacking flagella in a collection obtained from humans and cattle. The aflagellate strains were impaired in their proinflammatory properties and The aim of this work was to elucidate the underlying cause of the absence of flagella in Dublin isolates. We report here that class 3 flagellar genes are repressed in the human aflagellate isolates, due to impaired secretion of FliA anti-sigma factor FlgM. This phenotype is due to an in-frame 42-nucleotide deletion in the gene, which codes for a protein located in the flagellar basal body. The deletion is predicted to produce a protein lacking amino...
    Is part of: Infection and immunity, January 2018, Vol.86(1)
    Identifier: 1098-5522 (E-ISSN); 29061704 Version (PMID); 10.1128/IAI.00517-17 (DOI)