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    • Several versions

    Anti-Fading Media for Live Cell GFP Imaging

    Bogdanov, Alexey M, Kudryavtseva, Elena I, Lukyanov, Konstantin A
    PLoS ONE, 2012, Vol.7(12) [Peer Reviewed Journal]

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    Turning On and Off Photoinduced Electron Transfer in Fluorescent Proteins by π-Stacking, Halide Binding, and Tyr145 Mutations

    Bogdanov, Alexey M, Acharya, Atanu, Titelmayer, Anastasia V, Mamontova, Anastasia V, Bravaya, Ksenia B, Kolomeisky, Anatoly B, Lukyanov, Konstantin A, Krylov, Anna I
    Journal of the American Chemical Society, 13 April 2016, Vol.138(14), pp.4807-4817 [Peer Reviewed Journal]
    ACS Publications (American Chemical Society)
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    Title: Turning On and Off Photoinduced Electron Transfer in Fluorescent Proteins by π-Stacking, Halide Binding, and Tyr145 Mutations
    Author: Bogdanov, Alexey M; Acharya, Atanu; Titelmayer, Anastasia V; Mamontova, Anastasia V; Bravaya, Ksenia B; Kolomeisky, Anatoly B; Lukyanov, Konstantin A; Krylov, Anna I
    Subject: Chemistry
    Description: Photoinduced electron transfer in fluorescent proteins from the GFP family can be regarded either as an asset facilitating new applications or as a nuisance leading to the loss of optical output. Photooxidation commonly results in green-to-red photoconversion called oxidative redding. We discovered that yellow FPs do not undergo redding; however, the redding is restored upon halide binding. Calculations of the energetics of one-electron oxidation and possible electron transfer (ET) pathways suggested that excited-state ET proceeds through a hopping mechanism via Tyr145. In YFPs, the π-stacking of the chromophore with Tyr203 reduces its electron-donating ability, which can be restored by halide binding. Point mutations confirmed that Tyr145 is a key residue controlling ET. Substitution of Tyr145 by less-efficient electron acceptors resulted in highly photostable mutants. This strategy (i.e., calculation and disruption of ET pathways by mutations) may represent a new approach toward...
    Is part of: Journal of the American Chemical Society, 13 April 2016, Vol.138(14), pp.4807-4817
    Identifier: 0002-7863 (ISSN); 1520-5126 (E-ISSN); 10.1021/jacs.6b00092 (DOI)

    • Several versions

    Impact of methylations of m2G966/m5C967 in 16S rRNA on bacterial fitness and translation initiation

    Burakovsky, Dmitry E, Prokhorova, Irina V, Sergiev, Petr V, Milón, Pohl, Sergeeva, Olga V, Bogdanov, Alexey A, Rodnina, Marina V, Dontsova, Olga A
    Nucleic acids research, September 2012, Vol.40(16), pp.7885-95 [Peer Reviewed Journal]

    • Several versions

    Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor

    Kost, Liubov A, Nikitin, Evgeny S, Ivanova, Violetta O, Sung, Uhna, Putintseva, Ekaterina V, Chudakov, Dmitry M, Balaban, Pavel M, Lukyanov, Konstantin A, Bogdanov, Alexey M
    PloS one, 2017, Vol.12(9), pp.e0184225 [Peer Reviewed Journal]

    • Several versions

    Research of kinematics of an actuating group of an anthropomorphic gripper with a common drive

    Bogdanov Alexey, Permyakov Alexander, Zhdanova Yulia
    MATEC web of conferences, 01 January 2018, Vol.224, p.01029 [Peer Reviewed Journal]

    • Several versions

    Techniques for Screening Translation Inhibitors

    Osterman, Ilya A, Bogdanov, Alexey A, Dontsova, Olga A, Sergiev, Petr V
    Antibiotics, 2016, Vol.5(3) [Peer Reviewed Journal]

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    Orientation order and rotation mobility of nitroxide biradicals determined by quantitative simulation of EPR spectra

    Bogdanov, Alexey V, Vorobiev, Andrey Kh
    Physical chemistry chemical physics : PCCP, 16 November 2016, Vol.18(45), pp.31144-31153 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
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    Title: Orientation order and rotation mobility of nitroxide biradicals determined by quantitative simulation of EPR spectra
    Author: Bogdanov, Alexey V; Vorobiev, Andrey Kh
    Subject: Numerische Simulation ; Drehbewegung ; Ordnungsparameter ; Austausch ; Mobilität ; Flüssigkristall ; Elektron ; Molekulardynamik ; Lösungsmittel ; Chemistry;
    Description: The problem of quantitative numerical simulation of electron paramagnetic resonance (EPR) spectra of biradical probes in both isotropic and aligned media was solved for the first time. The models suitable for the description of the spectra of the probes, both in the rigid limit and in the presence of rotational motions, were developed and successfully applied to model systems. The simulation of EPR spectra allows obtaining the following information about the molecular structure and dynamics: the values of orientation order parameters, the type of rotation mobility and its quantitative characteristics, and the sign and value of the spin exchange constant of the biradical. Model systems used in this work include solutions of nitroxide biradicals in a viscous solvent (squalane) in the range of temperatures 100-370 K and in the aligned liquid crystal n-octylcyanobiphenyl (8CB, 100-298.5 K). Unexpectedly, it was found that in 8CB the main orientation axis of the biradical molecule is perpendicular...
    Is part of: Physical chemistry chemical physics : PCCP, 16 November 2016, Vol.18(45), pp.31144-31153
    Identifier: 1463-9084 (E-ISSN); 27812560 Version (PMID)

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    Uncontrollable haemorrhage following dabigatran prophylaxis

    Sandhu, Pavandeep Singh, Wilson, Caroline, Dalton, Emily, Bogdanov, Alexey
    BMJ case reports, 10 June 2013, Vol.2013 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
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    Title: Uncontrollable haemorrhage following dabigatran prophylaxis
    Author: Sandhu, Pavandeep Singh; Wilson, Caroline; Dalton, Emily; Bogdanov, Alexey
    Subject: Antithrombins -- Adverse Effects ; Benzimidazoles -- Adverse Effects ; Beta-Alanine -- Analogs & Derivatives
    Description: An 88-year-old man, on dabigatran anticoagulation after a total hip replacement 1 week before admission, presented with acute abdominal pain associated with malaena and haematemesis. After examination and investigation, he was found to have a perforated viscus and underwent laparotomy. The perforation was surgically repaired, but the patient suffered from a massive haemorrhage during the operation, requiring intensive care admission postoperatively. The patient subsequently deteriorated despite aggressive treatment and died several days later.
    Is part of: BMJ case reports, 10 June 2013, Vol.2013
    Identifier: 1757-790X (E-ISSN); 23761516 Version (PMID); 10.1136/bcr-2013-010249 (DOI)

    • Several versions

    Influence of cell growth conditions and medium composition on EGFP photostability in live cells

    Mamontova, Anastasia V, Bogdanov, Alexey M, Lukyanov, Konstantin A
    BioTechniques, 01 May 2015, Vol.58(5), pp.258-261 [Peer Reviewed Journal]

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    Magnetic field effects in nematic and smectic liquid crystals probed by time resolved observation of orientation relaxation of the spin probe

    Bogdanov, Alexey V, Proniuk, Gleb I, Vorobiev, Andrey Kh
    Physical chemistry chemical physics : PCCP, 11 July 2018, Vol.20(27), pp.18340-18347 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
    Available
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    Title: Magnetic field effects in nematic and smectic liquid crystals probed by time resolved observation of orientation relaxation of the spin probe
    Author: Bogdanov, Alexey V; Proniuk, Gleb I; Vorobiev, Andrey Kh
    Subject: Magnetic-Field ; Kinetics ; Distribution-Function ; Experimental-Data ; Nematic-Phases ; Nematic-Liquid-Crystals ; Magnetic-Field-Effect ; Format-Rearrangement ; Smectic-Phase ; Elastic-Modulus ; Magnetisches Feld ; Kinetik ; Verteilungsfunktion ; Experimentelle Daten ; Nematische Phase ; Nematischer Flüssigkristall ; Magnetischer Feldeffekt ; Formatumstellung ; Smektische Phase ; Elastizitätskonstante ; Chemistry;
    Description: The kinetics of reorientation of the liquid crystal HOPOOB in nematic and smectic phases in the magnetic field was studied by the spin probe technique. The time evolution of the EPR spectrum shape in the course of reorientation in the nematic phase indicates that the mechanism of alignment comprises redistribution between domains of different orientations rather than the turn of the orientation distribution function. A microscopic model of domain structure rearrangement is provided to qualitatively describe the experimental data. In the smectic C phase, partial reorientation is observed upon the action of the magnetic field. A possible mechanism of this process is the reorientation of tilt direction within the smectic layers. It is shown that this process is constrained, and the value of the corresponding elastic constant is estimated.
    Is part of: Physical chemistry chemical physics : PCCP, 11 July 2018, Vol.20(27), pp.18340-18347
    Identifier: 1463-9084 (E-ISSN); 29938286 Version (PMID); 10.1039/c8cp01753k (DOI)