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    • Several versions

    A novel prophage identified in strains from Salmonella enterica serovar Enteritidis is a phylogenetic signature of the lineage ST-1974

    D'Alessandro, Bruno, Pérez Escanda, Victoria, Balestrazzi, Lucía, Iriarte, Andrés, Pickard, Derek, Yim, Lucía, Chabalgoity, José Alejandro, Betancor, Laura
    Microbial genomics, March 2018, Vol.4(3) [Peer Reviewed Journal]

    • Several versions

    Proteomic analysis of Proteus mirabilis outer membrane proteins reveals differential expression in vivo vs. in vitro conditions

    D'Alessandro, Bruno, Lery, Leticia M. S, Von Krüger, Wanda M. A, Lima, Analía, Piccini, Claudia, Zunino, Pablo
    FEMS Immunology & Medical Microbiology, 2011, Vol. 63(2), pp.174-182 [Peer Reviewed Journal]

    • Several versions

    First Release of the Bacterial Biobank of the Urban Environment (BBUE)

    Antelo, Verónica, Salazar, Cecilia, Martínez, Arací, D’alessandro, Bruno, Castro, Marta, Betancor, Laura, Barcala, María Victoria, Míguez, Diana, Gonnet, Gastón H, Iraola, Gregorio
    Microbiology Resource Announcements, 2018, Vol.7(16) [Peer Reviewed Journal]

    • Several versions

    Draft Genome Sequences of Two Multidrug-Resistant Salmonella enterica Serovar Typhimurium Clinical Isolates from Uruguay

    Cordeiro, Nicolás F, D’alessandro, Bruno, Iriarte, Andrés, Pickard, Derek, Yim, Lucía, Chabalgoity, José Alejandro, Betancor, Laura, Vignoli, Rafael
    Microbiology Resource Announcements, 2018, Vol.7(4) [Peer Reviewed Journal]

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    A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties

    Sasías, Sebastián, Martínez-Sanguiné, Adriana, Betancor, Laura, Martínez, Arací, D'Alessandro, Bruno, Iriarte, Andrés, Chabalgoity, José A, Yim, Lucía
    Infection and immunity, January 2018, Vol.86(1) [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
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    Title: A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties
    Author: Sasías, Sebastián; Martínez-Sanguiné, Adriana; Betancor, Laura; Martínez, Arací; D'Alessandro, Bruno; Iriarte, Andrés; Chabalgoity, José A; Yim, Lucía
    Subject: Flie ; Salmonella ; Flagella ; Proinflammatory Capacity ; Serovar Dublin ; Bacterial Proteins -- Genetics ; Flagella -- Genetics ; Salmonella Enterica -- Genetics ; Sequence Deletion -- Genetics
    Description: serovar Dublin is adapted to cattle but is able to infect humans with high invasiveness. An acute inflammatory response at the intestine helps to prevent dissemination to systemic sites. Flagella contribute to this response by providing motility and FliC-mediated signaling through pattern recognition receptors. In a previous work, we reported a high frequency (11 out of 25) of Dublin isolates lacking flagella in a collection obtained from humans and cattle. The aflagellate strains were impaired in their proinflammatory properties and The aim of this work was to elucidate the underlying cause of the absence of flagella in Dublin isolates. We report here that class 3 flagellar genes are repressed in the human aflagellate isolates, due to impaired secretion of FliA anti-sigma factor FlgM. This phenotype is due to an in-frame 42-nucleotide deletion in the gene, which codes for a protein located in the flagellar basal body. The deletion is predicted to produce a protein lacking amino...
    Is part of: Infection and immunity, January 2018, Vol.86(1)
    Identifier: 1098-5522 (E-ISSN); 29061704 Version (PMID); 10.1128/IAI.00517-17 (DOI)

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    City-wide metagenomics uncover antibiotic resistance reservoirs in urban beach and sewage waters

    Fresia, Pablo, Antelo, Veronica, Salazar, Cecilia, Gimenez, Matias, D'Alessandro, Bruno, Afshinnekoo, Ebrahim, Mason, Christopher, Gonnet, Gaston, Iraola, Gregorio
    BioRxiv, Oct 30, 2018
    © ProQuest LLC All rights reserved, Biological Science Database, Publicly Available Content Database, ProQuest Biological Science Collection, ProQuest Natural Science Collection, ProQuest SciTech Collection, Natural Science Collection, ProQuest Central (new), ProQuest Central K-12, ProQuest Central Korea, SciTech Premium Collection, ProQuest Central Essentials, ProQuest One Academic
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    Title: City-wide metagenomics uncover antibiotic resistance reservoirs in urban beach and sewage waters
    Author: Fresia, Pablo; Antelo, Veronica; Salazar, Cecilia; Gimenez, Matias; D'Alessandro, Bruno; Afshinnekoo, Ebrahim; Mason, Christopher; Gonnet, Gaston; Iraola, Gregorio
    Contributor: Iraola, Gregorio (pacrepositoryorg)
    Subject: Pathogens ; Exploration ; Public Health ; Epidemiology ; Antibiotics ; Virulence ; Public Health ; Coastal Environments ; Antibiotic Resistance ; Genotypes ; Beaches ; Pathogens ; Drug Resistance ; Sewage ; Health Surveillance
    Description: Background: Microbial communities present in environmental waters constitute a reservoir for antibiotic resistant pathogens that impact human health. For this reason a diverse variety of water environments are being analyzed using metagenomics to uncover public health threats. However, the composition of these communities along the coastal environment of a whole city where sewage and beach waters are mixed, is poorly understood. Results: We shotgun-sequenced 20 coastal areas from the city of Montevideo (capital of Uruguay) including beach and sewage water samples to characterize bacterial communities and their virulence and antibiotic resistance repertories. We found that sewage and beach environments presented significantly different bacterial communities. Sewage waters harbored a higher prevalence and a more diverse repertory of virulence and antibiotic resistant genes mainly from well-known enterobacteria, including carbapenemases and extended-spectrum betalactamases reported in hospital...
    Is part of: BioRxiv, Oct 30, 2018
    Identifier: 10.1101/456517 (DOI)

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    First release of the bacterial biobank of the urban environment (BBUE)

    Antelo, Verónica, Salazar, Cecilia, Martínez, Arací, D’alessandro, Bruno, Castro, Marta, Betancor, Laura, Barcala, María V, Míguez, Diana, Gonnet, Gastón H, Iraola, Gregorio
    Microbiology Resource Announcements, 2018
    Research Collection (ETH Zurich)
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    Title: First release of the bacterial biobank of the urban environment (BBUE)
    Author: Antelo, Verónica; Salazar, Cecilia; Martínez, Arací; D’alessandro, Bruno; Castro, Marta; Betancor, Laura; Barcala, María V; Míguez, Diana; Gonnet, Gastón H; Iraola, Gregorio
    Description: Metagenomics is providing a broad overview of bacterial functional diversity; however, culturing and biobanking are still essential for microbiology. Here, we present the Bacterial Biobank of the Urban Environment (BBUE), a sizable culture collection for long-term storage and characterization of the microbiota associated with urban environments relevant for public health.
    Is part of: Microbiology Resource Announcements, 2018
    Identifier: 10.3929/ethz-b-000304430 (DOI)

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    Detection of Paenibacillus larvae subspecies larvae spores in naturally infected bee larvae and artificially contaminated honey by PCR

    Piccini, Claudia, D'Alessandro, Bruno, Antúnez, Karina, Zunino, Pablo
    World Journal of Microbiology and Biotechnology, 2002, Vol.18(8), pp.761-765 [Peer Reviewed Journal]
    Springer Science & Business Media B.V.
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    Title: Detection of Paenibacillus larvae subspecies larvae spores in naturally infected bee larvae and artificially contaminated honey by PCR
    Author: Piccini, Claudia; D'Alessandro, Bruno; Antúnez, Karina; Zunino, Pablo
    Subject: American foulbrood ; American foulbrood-contaminated honey ; dead bee larvae ; subspecies ; polymerase chain reaction ; 16S rRNA ; spore detection
    Description: American foulbrood (AFB), a severe bacterial disease of honeybee brood, has recently been found in Uruguayan apiaries. Detection of the causative agent, Paenibacillus larvae subspecies larvae , is a very important concern in order to prevent disease dissemination and decrease of honey production. Since spores are the infective forms of this pathogen, in the present work we report the use of polymerase chain reaction (PCR) to detect P. l. subsp. larvae spores from in vitro cultures, larvae with clinical symptoms and experimentally contaminated honey. The set of primers was designed based on the published P. l. subsp. larvae 16S rRNA gene. Using this approach we could amplify the pathogen DNA and obtain a great sensitivity and a notable specificity. Detection limit for spore suspension was a 10 −2 dilution of template DNA obtained from 32 spores, as determined by plate count. For artificially contaminated honey, we could detect the PCR product at a 10 −3 dilution of template DNA obtained from 170 spores. In addition, when PCR conditions were set to improve specificity, we were able to amplify P. l . subsp. larvae DNA selectively and no cross-reactions were observed with a variety of related bacterial species, including P. l. subsp. pulvifaciens . Since spore detection is very important to confirm the presence of the disease, this method provides a reliable diagnosis of AFB from infected larvae and contaminated honey in a few hours.
    Is part of: World Journal of Microbiology and Biotechnology, 2002, Vol.18(8), pp.761-765
    Identifier: 0959-3993 (ISSN); 1573-0972 (E-ISSN); 10.1023/A (DOI)

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    An iron-regulated outer-membrane protein of Proteus mirabilis is a haem receptor that plays an important role in urinary tract infection and in in vivo growth

    Lima, Analía, Zunino, Pablo, D'Alessandro, Bruno, Piccini, Claudia
    Journal of medical microbiology, December 2007, Vol.56(Pt 12), pp.1600-1607 [Peer Reviewed Journal]
    MEDLINE/PubMed (U.S. National Library of Medicine)
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    Title: An iron-regulated outer-membrane protein of Proteus mirabilis is a haem receptor that plays an important role in urinary tract infection and in in vivo growth
    Author: Lima, Analía; Zunino, Pablo; D'Alessandro, Bruno; Piccini, Claudia
    Subject: Bacterial Outer Membrane Proteins -- Metabolism ; Iron -- Pharmacology ; Proteus Mirabilis -- Metabolism ; Receptors, Cell Surface -- Metabolism ; Urinary Tract Infections -- Physiopathology
    Description: Proteus mirabilis, a common cause of urinary tract infections, expresses iron-regulated outer-membrane proteins (OMPs) in response to iron restriction. It has been suggested that a 64 kDa OMP is involved in haemoprotein uptake and that this might have a role in pathogenesis. In order to confirm this hypothesis, this study generated a P. mirabilis mutant strain (P7) that did not express the 64 kDa OMP, by insertion of the TnphoA transposon. The nucleotide sequence of the interrupted gene revealed that it corresponded to a haemin receptor precursor. Moreover, in vitro growth assays showed that the mutant was unable to grow using haemoglobin and haemin as unique iron sources. The authors also carried out in vivo growth and infectivity assays and demonstrated that P7 was not able to survive in an in vivo model and was less efficient than wild-type strain Pr 6515 in colonizing the urinary tract. These results confirmed that the P. mirabilis 64 kDa iron-regulated OMP is a haem receptor that has...
    Is part of: Journal of medical microbiology, December 2007, Vol.56(Pt 12), pp.1600-1607
    Identifier: 0022-2615 (ISSN); 18033826 Version (PMID); 10.1099/jmm.0.47320-0 (DOI)

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    DNA extraction and PCR detection of Paenibacillus larvae spores from naturally contaminated honey and bees using spore-decoating and freeze-thawing techniques

    D’Alessandro, Bruno, Antúnez, Karina, Piccini, Claudia, Zunino, Pablo
    World Journal of Microbiology and Biotechnology, 2007, Vol.23(4), pp.593-597 [Peer Reviewed Journal]
    Springer Science & Business Media B.V.
    Available
    More…
    Title: DNA extraction and PCR detection of Paenibacillus larvae spores from naturally contaminated honey and bees using spore-decoating and freeze-thawing techniques
    Author: D’Alessandro, Bruno; Antúnez, Karina; Piccini, Claudia; Zunino, Pablo
    Subject: American foulbrood ; Paenibacillus larvae ; Spores ; Spore-decoating ; Freeze-thawing ; Polymerase chain reaction
    Description: Paenibacillus larvae causes American foulbrood (AFB), a severe disease that affects the brood of honey bee Apis mellifera. AFB is worldwide distributed and causes great economic losses to beekeepers, but in many cases early diagnosis could help in its prevention and control. The aim of the present work was to design a reliable protocol for DNA extraction of P. larvae spores from naturally contaminated honey and adult bees. A novel method that includes a step of spore-decoating followed by an enzymatic spore disruption and DNA purification was developed. Also a freeze-thaw cycle protocol was tested and the results were compared. The DNA extracted was used as template for specific bacterial detection by amplification of a 16S rDNA fragment. Both methods allowed the direct detection by polymerase chain reaction (PCR) of P. larvae spores present in naturally contaminated material. The spore-decoating strategy was the most successful method for DNA extraction from spores, allowing specific and remarkably sensitive PCR detection of spores in all honey and bees tested samples. On the other hand freeze-thawing was only effective for detection of spores recovered from bees, and extensive damage to DNA affected detection by PCR. This work provides new strategies for spore DNA extraction and detection by PCR with high sensitivity, and brings an alternative tool for P. larvae detection in natural samples.
    Is part of: World Journal of Microbiology and Biotechnology, 2007, Vol.23(4), pp.593-597
    Identifier: 0959-3993 (ISSN); 1573-0972 (E-ISSN); 10.1007/s11274-006-9261-y (DOI)