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    • Several versions

    Further insights into the tRNA modification process controlled by proteins MnmE and GidA of Escherichia coli

    Yim, Lucía, Moukadiri, Ismaïl, Björk, Glenn R, Armengod, M-Eugenia
    Nucleic acids research, 2006, Vol.34(20), pp.5892-905 [Peer Reviewed Journal]

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    Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

    Silva, Claudia, Betancor, Laura, García, Coralith, Astocondor, Lizeth, Hinostroza, Noemí, Bisio, Julieta, Rivera, Javier, Perezgasga, Lucía, Pérez Escanda, Victoria, Yim, Lucía, Jacobs, Jan, García-Del Portillo, Francisco, Chabalgoity, José A
    PLoS ONE, 2017, Vol.12(12) [Peer Reviewed Journal]

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    Synthesis of metallo-β-lactamase VIM-2 is associated with a fitness reduction in Salmonella enterica Serovar Typhimurium

    Cordeiro, Nicolás F, Chabalgoity, José A, Yim, Lucía, Vignoli, Rafael
    Antimicrobial agents and chemotherapy, November 2014, Vol.58(11), pp.6528-35 [Peer Reviewed Journal]
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    Title: Synthesis of metallo-β-lactamase VIM-2 is associated with a fitness reduction in Salmonella enterica Serovar Typhimurium
    Author: Cordeiro, Nicolás F; Chabalgoity, José A; Yim, Lucía; Vignoli, Rafael
    Subject: Drug Resistance, Multiple, Bacterial -- Genetics ; Genetic Fitness -- Genetics ; Salmonella Infections -- Drug Therapy ; Salmonella Typhimurium -- Pathogenicity ; Beta-Lactamases -- Genetics
    Description: Antibiotic resistance, especially due to β-lactamases, has become one of the main obstacles in the correct treatment of Salmonella infections; furthermore, antibiotic resistance determines a gain of function that may encompass a biological cost, or fitness reduction, to the resistant bacteria. The aim of this work was to determine in vitro if the production of the class B β-lactamase VIM-2 determined a fitness cost for Salmonella enterica serovar Typhimurium. To that end the gene blaVIM-2 was cloned into the virulent strain S. Typhimurium SL1344, using both the tightly regulated pBAD22 vector and the natural plasmid pST12, for inducible and constitutive expression, respectively. Fitness studies were performed by means of motility, growth rate, invasiveness in epithelial cells, and plasmid stability. The expression of blaVIM-2 was accompanied by alterations in micro- and macroscopic morphology and reduced growth rate and motility, as well as diminished invasiveness in epithelial cells. These...
    Is part of: Antimicrobial agents and chemotherapy, November 2014, Vol.58(11), pp.6528-35
    Identifier: 1098-6596 (E-ISSN); 25136026 Version (PMID); 10.1128/AAC.02847-14 (DOI)

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    Naturally occurring motility-defective mutants of Salmonella enterica serovar Enteritidis isolated preferentially from nonhuman rather than human sources

    Yim, Lucía, Betancor, Laura, Martínez, Arací, Bryant, Clare, Maskell, Duncan, Chabalgoity, José A
    Applied and environmental microbiology, November 2011, Vol.77(21), pp.7740-8 [Peer Reviewed Journal]
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    Title: Naturally occurring motility-defective mutants of Salmonella enterica serovar Enteritidis isolated preferentially from nonhuman rather than human sources
    Author: Yim, Lucía; Betancor, Laura; Martínez, Arací; Bryant, Clare; Maskell, Duncan; Chabalgoity, José A
    Subject: Locomotion ; Salmonella Infections -- Microbiology ; Salmonella Infections, Animal -- Microbiology ; Salmonella Enteritidis -- Isolation & Purification
    Description: Salmonellosis represents a worldwide health problem because it is one of the major causes of food-borne disease. Although motility is postulated as an important Salmonella virulence attribute, there is little information about variation in motility in natural isolates. Here we report the identification of a point mutation (T551 → G) in motA, a gene essential for flagellar rotation, in several Salmonella enterica serovar Enteritidis field isolates. This mutation results in bacteria that can biosynthesize structurally normal but paralyzed flagella and are impaired in their capacity to invade human intestinal epithelial cells. Introduction of a wild-type copy of motA into one of these isolates restored both motility and cell invasiveness. The motA mutant triggered higher proinflammatory transcriptional responses than an aflagellate isolate in differentiated Caco-2 cells, suggesting that the paralyzed flagella are able to signal through pattern recognition receptors. A specific PCR was designed...
    Is part of: Applied and environmental microbiology, November 2011, Vol.77(21), pp.7740-8
    Identifier: 1098-5336 (E-ISSN); 21926214 Version (PMID); 10.1128/AEM.05318-11 (DOI)

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    A novel prophage identified in strains from Salmonella enterica serovar Enteritidis is a phylogenetic signature of the lineage ST-1974

    D'Alessandro, Bruno, Pérez Escanda, Victoria, Balestrazzi, Lucía, Iriarte, Andrés, Pickard, Derek, Yim, Lucía, Chabalgoity, José Alejandro, Betancor, Laura
    Microbial genomics, March 2018, Vol.4(3) [Peer Reviewed Journal]

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    Repression of flagella is a common trait in field isolates of Salmonella enterica serovar Dublin and is associated with invasive human infections

    Yim, Lucía, Sasías, Sebastián, Martínez, Arací, Betancor, Laura, Estevez, Verónica, Scavone, Paola, Bielli, Alejandro, Sirok, Alfredo, Chabalgoity, José Alejandro
    Infection and immunity, April 2014, Vol.82(4), pp.1465-76 [Peer Reviewed Journal]
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    Title: Repression of flagella is a common trait in field isolates of Salmonella enterica serovar Dublin and is associated with invasive human infections
    Author: Yim, Lucía; Sasías, Sebastián; Martínez, Arací; Betancor, Laura; Estevez, Verónica; Scavone, Paola; Bielli, Alejandro; Sirok, Alfredo; Chabalgoity, José Alejandro
    Subject: Flagella -- Physiology ; Salmonella Infections -- Microbiology ; Salmonella Enterica -- Pathogenicity
    Description: The nontyphoidal Salmonella enterica serovar Dublin is adapted to cattle but infrequently infects humans, very often resulting in invasive infections with high levels of morbidity and mortality. A Salmonella-induced intestinal acute inflammatory response is postulated as a mechanism to prevent bacterial dissemination to systemic sites. In S. enterica serovar Typhimurium, flagella contribute to this response by providing motility and FliC-mediated activation of pattern recognition receptors. In this study, we found 4 Salmonella enterica isolates, with the antigenic formula 9,12:-:-, that, based on fliC sequence and multilocus sequence type (MLST) analyses, are aflagellate S. Dublin isolates. Interestingly, all were obtained from human bloodstream infections. Thus, we investigated the potential role of flagella in the unusual invasiveness exhibited by S. Dublin in humans by analyzing flagellation and proinflammatory properties of a collection of 10 S. Dublin human clinical isolates. We found...
    Is part of: Infection and immunity, April 2014, Vol.82(4), pp.1465-76
    Identifier: 1098-5522 (E-ISSN); 24421045 Version (PMID); 10.1128/IAI.01336-13 (DOI)

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    Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica

    Hiriart, Yanina, Serradell, Maria, Martínez, Araci, Sampaolesi, Sofia, Maciel, Dolores Gonzalez, Chabalgoity, Jose Alejandro, Yim, Lucía, Algorta, Gabriela, Rumbo, Martin
    SpringerPlus, 2013, Vol.2, pp.640 [Peer Reviewed Journal]
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    Title: Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
    Author: Hiriart, Yanina; Serradell, Maria; Martínez, Araci; Sampaolesi, Sofia; Maciel, Dolores Gonzalez; Chabalgoity, Jose Alejandro; Yim, Lucía; Algorta, Gabriela; Rumbo, Martin
    Subject: Flagellin ; Monoclonal Antibodies ; Salmonella ; Serotyping
    Description: In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb...
    Is part of: SpringerPlus, 2013, Vol.2, pp.640
    Identifier: 2193-1801 (ISSN); 24349948 Version (PMID); 10.1186/2193-1801-2-640 (DOI)

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    Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin

    Betancor, Laura, Yim, Lucía, Martínez, Arací, Fookes, Maria, Sasias, Sebastian, Schelotto, Felipe, Thomson, Nicholas, Maskell, Duncan, Chabalgoity, José A
    The open microbiology journal, 2012, Vol.6, pp.5-13 [Peer Reviewed Journal]
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    Title: Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin
    Author: Betancor, Laura; Yim, Lucía; Martínez, Arací; Fookes, Maria; Sasias, Sebastian; Schelotto, Felipe; Thomson, Nicholas; Maskell, Duncan; Chabalgoity, José A
    Subject: Comparative Genomics ; Host Specificity ; Pseudogenes ; S. Dublin ; S. Enteritidis. ; Salmonella
    Description: The Enteritidis and Dublin serovars of Salmonella enterica are closely related, yet they differ significantly in pathogenicity and epidemiology. S. Enteritidis is a broad host range serovar that commonly causes gastroenteritis and infrequently causes invasive disease in humans. S. Dublin mainly colonizes cattle but upon infecting humans often results in invasive disease.To gain a broader view of the extent of these differences we conducted microarray-based comparative genomics between several field isolates from each serovar. Genome degradation has been correlated with host adaptation in Salmonella, thus we also compared at whole genome scale the available genomic sequences of them to evaluate pseudogene composition within each serovar.Microarray analysis revealed 3771 CDS shared by both serovars while 33 were only present in Enteritidis and 87 were exclusive to Dublin. Pseudogene evaluation showed 177 inactive CDS in S. Dublin which correspond to active genes in S. Enteritidis, nine of...
    Is part of: The open microbiology journal, 2012, Vol.6, pp.5-13
    Identifier: 1874-2858 (E-ISSN); 22371816 Version (PMID); 10.2174/1874285801206010005 (DOI)

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    Draft Genome Sequences of Two Multidrug-Resistant Salmonella enterica Serovar Typhimurium Clinical Isolates from Uruguay

    Cordeiro, Nicolás F, D’alessandro, Bruno, Iriarte, Andrés, Pickard, Derek, Yim, Lucía, Chabalgoity, José Alejandro, Betancor, Laura, Vignoli, Rafael
    Microbiology Resource Announcements, 2018, Vol.7(4) [Peer Reviewed Journal]

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    A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties

    Sasías, Sebastián, Martínez-Sanguiné, Adriana, Betancor, Laura, Martínez, Arací, D'Alessandro, Bruno, Iriarte, Andrés, Chabalgoity, José A, Yim, Lucía
    Infection and immunity, January 2018, Vol.86(1) [Peer Reviewed Journal]
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    Title: A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties
    Author: Sasías, Sebastián; Martínez-Sanguiné, Adriana; Betancor, Laura; Martínez, Arací; D'Alessandro, Bruno; Iriarte, Andrés; Chabalgoity, José A; Yim, Lucía
    Subject: Flie ; Salmonella ; Flagella ; Proinflammatory Capacity ; Serovar Dublin ; Bacterial Proteins -- Genetics ; Flagella -- Genetics ; Salmonella Enterica -- Genetics ; Sequence Deletion -- Genetics
    Description: serovar Dublin is adapted to cattle but is able to infect humans with high invasiveness. An acute inflammatory response at the intestine helps to prevent dissemination to systemic sites. Flagella contribute to this response by providing motility and FliC-mediated signaling through pattern recognition receptors. In a previous work, we reported a high frequency (11 out of 25) of Dublin isolates lacking flagella in a collection obtained from humans and cattle. The aflagellate strains were impaired in their proinflammatory properties and The aim of this work was to elucidate the underlying cause of the absence of flagella in Dublin isolates. We report here that class 3 flagellar genes are repressed in the human aflagellate isolates, due to impaired secretion of FliA anti-sigma factor FlgM. This phenotype is due to an in-frame 42-nucleotide deletion in the gene, which codes for a protein located in the flagellar basal body. The deletion is predicted to produce a protein lacking amino...
    Is part of: Infection and immunity, January 2018, Vol.86(1)
    Identifier: 1098-5522 (E-ISSN); 29061704 Version (PMID); 10.1128/IAI.00517-17 (DOI)