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Titre: Comparison of Ultrastructure of Germinating Pea Leaves Prepared by High-Pressure Freezing-Freeze Substitution and Conventional Chemical Fixation Auteur:Kaneko, Yasuko Contributeur:Walther, Paul Sujet:Full-length Papers
- high-pressure freezing
- freeze substitution
- cryofixation
- chemical fixation
- Pisum sativum Description:
High-pressure freezing-freeze substitution (HPF-FS) methods were applied to germinating pea leaves. Good ultrastructural preservation without visible freezing damage was obtained up to 200 μm in thickness. Compared to conventional chemical fixation (CF), cellular membranes were smoother without undulation, and organelles appeared more turgid. The matrices of cytoplasm and organelles were denser and more homogeneous. These features imply that HPF-FS samples retain more substances and ultrastructure closer to the living state. There were differences in membrane stainability among organelles in HPF-FS specimens, which were not seen after CF. Bundles of microfilaments were observed frequently after HPF-FS
Publication en relation:
Microscopy. - 1995/44/2/104-109
Document hôte:Microscopy Identifiant:
10.1093/oxfordjournals.jmicro.a051149 (DOI)
Electron tomography reveals the fibril structure and lipid interactions in amyloid deposits
Kollmer, Marius, Meinhardt, Katrin, Haupt, Christian, Liberta, Falk, Wulff, Melanie, Linder, Julia, Handl, Lisa, Heinrich, Liesa, Loos, Cornelia, Schmidt, Matthias, Syrovets, Tatiana, Simmet, Thomas, Westermark, Per, Westermark, Gunilla T, Horn, Uwe, Schmidt, Volker, Walther, Paul, Fändrich, Marcus
Proceedings of the National Academy of Sciences of the United States of America, 17 May 2016, Vol.113(20), pp.5604-9
[Revue évaluée par les pairs]
Titre: Impact of Apple Rust Mite (Acari: Eriophyiidae) Feeding on Apple Leaf Gas Exchange and Leaf Color Associated with Changes in Leaf Tissue Auteur:Spieser, Fredy Contributeur:Graf, Benno; Walther, Paul; Noesberger, Josef Sujet:Community and Ecosystem Ecology
- Aculus schlechtendali
- apple
- photosynthesis
- transpiration
- leaf injury
- Eriophyiidae Description:
The impact of the apple rust mite, Aculus schlechtendali (Nalepa), on net CO2 exchange, transpiration rate, and leaf color of field-grown Jonagold and Golden Delicious apples was investigated. Apple rust mite feeding causes leaf browning. Changes in leaf color were measured to assess the cumulative leaf damage. Significant negative relationships were found between cumulative leaf damage and single-leaf net CO2 exchange as well as transpiration rate. The same trends were observed on both varieties, but the effect of apple rust mite feeding was more severe on Jonagold than on Golden Delicious. Leaf tissue injury was analyzed by cryoscanning electron microscopy and light microscopy. The pictures show that apple rust mites penetrate epidermal cells with their stylets, causing multiple puncture wounds. On heavily infested leaves, apple rust mite feeding causes desiccation of the epidermis and the spongy parenchyma. The resulting malfunction of the stomata and problems in gas exchange within the spongy parenchyma are likely to be the main reason for the reduction of gas exchange
Publication en relation:
Environmental Entomology. - 1998/27/5/1149-1156
Document hôte:Environmental Entomology Identifiant:
10.1093/ee/27.5.1149 (DOI)
Titre: Einführung in das Studium der indogermanischen Sprachwissenschaft, mit besonderer Berücksichtigung der klassischen und germanischen Sprachen Auteur:Josef, 1869-1938,Fischer, Walther Paul, 1889- Schrijnen Editeur:
Heidelberg, C. Winter
Date:
1921
Plusieurs versions
A Beta-Herpesvirus with Fluorescent Capsids to Study Transport in Living Cells (Imaging CMV in Live Cells)
A Luciferase Gene Driven by an Alphaherpesviral Promoter Also Responds to Immediate Early Antigens of the Betaherpesvirus HCMV, Allowing Comparative Analyses of Different Human Herpesviruses in One Reporter Cell Line
Anna Katharina Maier, Raimund Jung, Clarissa Villinger, Axel Schubert, Paul Walther, Christian Sinzger, Diana Lieber
PloS one, 01 January 2017, Vol.12(1), p.e0169580
[Revue évaluée par les pairs]
Metabolic Characterization of Intact Cells Reveals Intracellular Amyloid Beta but Not Its Precursor Protein to Reduce Mitochondrial Respiration
Patrick M Schaefer, Bjoern Von Einem, Paul Walther, Enrico Calzia, Christine A F Von Arnim
PloS one, 01 January 2016, Vol.11(12), p.e0168157
[Revue évaluée par les pairs]